Batman: Death and the Maidens #9 (2004)

Death and the Maidens, Chapter Nine
Nyssa's machinations have flourished, as she finally murders her father... Ra's al Ghul. And in doing so, she has become him. The tribe of Ubu serves her now, and while they quickly overpower Batman, he's left alive in the desert with the body of his nemesis. Now Ra's daughters sit united at the head of the League of Assassins, as Nyssa promises Bruce that she won't make her father's mistakes regarding him.
Release Date: 2004