Ultimate Spider-Man, Vol. 1 #13 (2001)

Confessions
Peter decides to come clean and tell Mary Jane everything. She doesn't believe him at first, so he's forced to put on a more convincing demonstration, complete with wall crawling. Mary Jane is understandably exciting, and jumps on the bed a bit herself. As they're starting to get closer, Aunt May interrupts. From her perspective, a teenage boy in her care was locked in a bedroom with a teenage girl. The sounds of whispers and bed squeaks come from the room, and May gets a very different impression of what is actually transpiring.
Release Date: 2001